Why is it important not to dilute the initial sample before it has been loaded

Just why is it crucial that you perhaps not dilute a short test before it
hasbeen packed on the chromatography line?

Major reason that, tall focused test is required for
line chromotography, after that we could observe good
(good levels), when we dilute the test and packed on line
causes unequal split, test are going to be dispersed within the
adsorbent and won’t develop good levels.

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